CLDF Title
Home | Contact Us | Bookmark
HBV HE HCC HCV
About CLDF Centers of Educational Expertise  
CME Dinner Meetings Telewebs Webcasts Slide Library Abstract Library Conference Highlights
 
Back  
 
Reuters Health Information (2006-06-02): Islet neogenesis reverses diabetes in mice: primate studies planned

Science

Islet neogenesis reverses diabetes in mice: primate studies planned

Last Updated: 2006-06-02 18:26:03 -0400 (Reuters Health)

NEW YORK (Reuters Health) - In diabetic mice, in vivo delivery of the neurogenin 3 (Ngn3) and betacellulin (BtC) genes induces liver cells to reprogram into mature islets containing glycemia-regulated, insulin-producing beta cells that restore normoglycemia and reverse diabetes.

Dr. Lawrence Chan from Baylor College of Medicine in Houston presented the research Friday at the 9th annual meeting of the American Society of Gene Therapy in Baltimore, Maryland.

Ngn3 is "an islet lineage defining transcription factor ... sufficient and necessary for normal islet development," Dr. Chan and colleagues explain in meeting materials. BtC is an islet growth factor.

In diabetic mice, a single injection of Ngn3 and BtC, using a helper-dependent adenoviral vector, restored normoglycemia within one week and this persisted for at least nine weeks, Dr. Chan reported.

Quantitative reverse-transcriptase polymerase chain reaction showed the presence of the islet hormones Ins1, Ins2, and glucagon in the livers of treated mice, and immunostaining revealed clusters of insulin-positive cells next to glucagon-positive cells, as seen in mature islets.

The current studies build on previous research by Dr. Chan's group in which they successfully reversed diabetes in mice by transferring BtC and the islet transcription factor NeuroD.

"With this earlier approach, we cured the diabetes and the animals had islet-like structures in the liver," Dr. Chan noted in an interview with Reuters Health, but the islets were not completely "normal." With Ngn3 and BtC, "the islets are much more normal," Dr. Chan said, "and the response was much better this time because we reversed diabetes within one week -- last time it took three weeks."

Equally important, Dr. Chan said, "In the earlier study, we did not know what cells were transformed into islet cells. We did not know whether they came from bone marrow lodged in the liver or came from some other types of cells. Now we have pretty good evidence that many of them were actually liver cells so we actually changed hepatocytes into islets or beta cells that produce insulin."

All of these observations, he said, set the stage for the next phase of studies in monkeys.

 
 
 
 
                 
 
HBV
Webcasts
Slide Library
Abstract Library
 
HE
CME Dinner Meeting
Webcasts
Slide Library
Abstract Library
 
HCC
Slide Library
Abstract Library
 
 
HCV
Webcasts
Slide Library
Abstract Library
 
CLDF Follow Us
   
 
About CLDF
Mission Statement
Board of Trustees
Board of Advisors
CLDF Supporters
 
Other Resources
Liver News Library
Journal Abstracts
Hep C Link to Care
 
Centers of
Educational Expertise
Regional Map
     
   
  The Chronic Liver Disease Foundation is a non-profit organization with content developed specifically for healthcare professionals.
© Copyright 2012-2014 Chronic Liver Disease Foundation. All rights reserved. This site is maintained as an educational resource for US healthcare providers only.
Use of this Web site is governed by the Chronic Liver Disease Foundation terms of use and privacy statement.