Institute of Digestive Disease and The Chinese University of Hong Kong, Hong Kong SAR, China; Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Hong Kong SAR, China.
Alpha-fetoprotein (AFP) is the most widely used biomarker for hepatocellular carcinoma (HCC) surveillance, which is criticized as neither sensitive nor specific in active hepatitis and liver cirrhosis. The aim of this study was to determine the performance of AFP as a tumor marker for HCC in entecavir-treated patients with chronic hepatitis B (CHB). This was a retrospective-prospective cohort study of 1,531 entecavir-treated patients under regular HCC surveillance with AFP and ultrasonography. The mean age was 52±12 years; 1,099 (72%) patients were male and 332 (21.7%) patients had clinical evidence of cirrhosis. At a mean follow-up of 51±13 months, 57 (2.9%) patients developed HCC of median size of 3.3cm. AFP fluctuated with alanine aminotransferase (ALT) and peaked at the time of starting entecavir, then gradually decreased afterwards. AFP started to increase 6 months before the diagnosis of HCC. The receiver operator characteristic curve (AUROC) of AFP was highest at time of HCC diagnosis (0.85, 95% confidence interval [CI]: 0.73-0.98), and remained satisfactory at 3 months (0.82, 95% CI: 0.73-0.91) and 6 months (0.79, 95% CI: 0.69-0.89) before the diagnosis. Using the conventional AFP cutoff (20 μg/l) at month 0, the sensitivity and specificity to diagnose HCC were 38.6% and 98.9% respectively. Adopting the lower cutoff value (6 μg/l) of AFP level at month 0, the sensitivity was increased to 80.7%, while the specificity was decreased to 80.4%.
On-treatment AFP is a specific tumor marker for HCC in CHB patients receiving entecavir therapy. Adopting a lower cutoff value of AFP level at 6 μg/l would increase the sensitivity significantly for HCC detection.